Question
Posted on: March 6 2013An ophthalmologist in our hospital has asked us to complete EDTA and sodium bicarbonate in vials of 20ml. How can we prepare this sterial?
This preparation should be sterile, as it serves to dissolve lime deposits in the eye. Sodium bicarbonate decomposes during heating and cannot be sterilized in the drying oven or autoclave. If Aseptic is worked (sterile mortar, pestle, spatula, vials and rubber caps) The powder is not yet sterile (after all, water molecules can be admaded on the fabrics). Is this a sufficiently correct procedure or is there a better method for sterile getting this preparation?
Answer
If you mean as a powder I see no method but why the solution does not make ex tempore and then there can be sterilized by fiiltration in a sterile vial. If we focus on perfusion fluids with NaHCO3 (I thought there is a solution with only NaHCO3?) then we can ascertain the shelf life of this solution. NaEDTA will not change much to this term.